Improving squalene production by enhancing the NADPH/NADP⁺ ratio, modifying the isoprenoid-feeding module and blocking the menaquinone pathway in Escherichia coli.

ABSTRACT:

Background

Squalene is currently used widely in the food, cosmetics, and medicine industries. It could also replace petroleum as a raw material for fuels. Microbial fermentation processes for squalene production have been emerging over recent years. In this study, to study the squalene-producing potential of Escherichia coli (E. coli), we employed several increasing strategies for systematic metabolic engineering. These include the expression of human truncated squalene synthase, the overexpression of rate-limiting enzymes in isoprenoid pathway, the modification of isoprenoid-feeding module and the blocking of menaquinone pathway.

Results

Herein, human truncated squalene synthase was engineered in Escherichia coli to create a squalene-producing bacterial

SUBMITTER: Xu W 

PROVIDER: S-EPMC6437923 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature