RNA N6-methyladenosine demethylase FTO promotes breast tumor progression through inhibiting BNIP3.

ABSTRACT:

Background

N6-methyladenosine (m6A) modification is the most pervasive modification in mRNA, and has been considered as a new layer of epigenetic regulation on mRNA processing, stability and translation. Despite its functional significance in various physiological processes, the role of the m6A modification involved in breast cancer is yet fully understood.

Methods

We used the m6A-RNA immunoprecipitation sequencing to identify the potential targets in breast cancer. To determine the underlying mechanism for the axis of FTO-BNIP3, we performed a series of in vitro and in vivo assays in 3 breast cancer cell lines and 36 primary breast tumor tissues and 12 adjunct tissues.

Results

We showed that FTO, a key m6A demethylase, was up-regulated in human breast cancer. High le

SUBMITTER: Niu Y 

PROVIDER: S-EPMC6437932 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature