Comparative cytotoxicity of respirable surface-treated/untreated calcium carbonate rock dust particles in vitro.

ABSTRACT: Calcium carbonate rock dust (RD) is used in mining to reduce the explosivity of aerosolized coal. During the dusting procedures, potential for human exposure occurs, raising health concerns. To improve RD aerosolization, several types of anti-caking surface treatments exist. The aim of the study was to evaluate cytotoxicity of four respirable RD samples: untreated/treated limestone (UL/TL), untreated/treated marble (UM/TM), and crystalline silica (SiO₂) as a positive control in A549 and THP-1 transformed human cell lines. Respirable fractions were generated and collected using FSP10 high flow-rate cyclone samplers. THP-1 cells were differentiated with phorbol-12-myristate-13-acetate (20 ng/ml, 48 h). Cells were exposed to seven different concentrations of RD and SiO₂

SUBMITTER: Khaliullin TO 

PROVIDER: S-EPMC6438615 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature