NDM-1 carbapenemase in Acinetobacter baumannii sequence type 32 in Ecuador.

ABSTRACT:

Objectives

To describe a clinical case of Acinetobacter baumannii sequence type (ST) 32 harbouring a New Delhi metallo-β-lactamase (NDM) in Ecuador.

Methods

We used multilocus sequence typing (MLST) to confirm the bacterial species and the sequence type of an A. baumannii isolate. We used synergy with the imipenem-EDTA disc method and the carbapenem inactivation method (CIM) to determine carbapenemase production; the presence of a carbapenemase gene was confirmed by PCR amplification and amplicon sequencing.

Results

Molecular characterization revealed the presence of A. baumannii ST32 harbouring the bla _NDM-1 gene in Ecuador. The bla _NDM-1 gene was isolated through PCR and amplified from a purified plasmi

SUBMITTER: Villacis JE 

PROVIDER: S-EPMC6438911 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature