CAMP Stimulates SLC26A3 Activity in Human Colon by a CFTR-Dependent Mechanism That Does Not Require CFTR Activity.

ABSTRACT:

Background & aims

SLC26A3 (DRA) is an electroneutral Cl^-/HCO₃^- exchanger that is present in the apical domain of multiple intestinal segments. An area that has continued to be poorly understood is related to DRA regulation in acute adenosine 3',5'-cyclic monophosphate (cAMP)-related diarrheas, in which DRA appears to be both inhibited as part of NaCl absorption and stimulated to contribute to increased HCO₃^- secretion. Different cell models expressing DRA have shown that cAMP inhibits, stimulates, or does not affect its activity.

Methods

This study re-evaluated cAMP regulation of DRA using new tools, including a successful knockout cell model, a specific DRA inhibitor (DRA_inh-A250), specific antibodies, and a

SUBMITTER: Tse CM 

PROVIDER: S-EPMC6438990 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature