Effect of intra-ovarian injection of mesenchymal stem cells in aged mares.

ABSTRACT:

Purpose

This study aims to determine if intra-ovarian injection of bone marrow-derived mesenchymal stem cells (MSCs) improves or restores ovarian function in aged females.

Methods

Prospective randomized study of eight aged mares and six young mares receiving intra-ovarian injection of MSCs or vehicle. Main outcome measures were antral follicle count and serum anti-Müllerian hormone (AMH) (aged and young mares), and for aged mares, oocyte meiotic and developmental competence; gross and histological ovarian assessment; evaluation of presence of chimerism in recovered granulosa cells and in ovarian tissue samples; and gene expression in ovarian tissue as assessed by RNA sequencing.

Results

Injection of MSCs was not associated with significant changes in follicle number,

SUBMITTER: Grady ST 

PROVIDER: S-EPMC6439078 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature