Dataset Information

RNA interference-mediated knockdown of voltage-gated sodium channel (MpNa_v) gene causes mortality in peach-potato aphid, Myzus persicae.

ABSTRACT: Voltage-gated sodium channels (VGSC) are transmembrane proteins that generate an action potential in excitable cells and play an essential role in neuronal signaling. Since VGSCs play a crucial role in nerve transmission they have become primary targets for a broad range of commercial insecticides. RNA interference (RNAi) is a valuable reverse genetics tool used in functional genomics, but recently, it has also shown promise as a novel agent that could be used to control agricultural insect pests. In this study, we targeted the VGSC (MpNa_v) gene in the peach-potato aphid Myzus persicae, by oral feeding of artificial diets mixed with dsRNAs. Knock-down of MpNa_v gene expression caused up to 65% mortality in 3^rd instar nymphs. Moreover, significantly lower fecundity and longevity was observed in adult aphids that had been fed with dsMpNa_v solution at the nymphal stage. Analysis of gene expression by qRT-PCR indicated that the aphid mortality rates and the lowered fecundity and longevity were attributable to the down-regulation of MpNa_v by RNAi. Taken together, our results show that MpNa_v is a viable candidate target gene for the development of an RNAi-based bio-aphicide.

SUBMITTER: Tariq K

PROVIDER: S-EPMC6439219 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature

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RNA interference-mediated knockdown of voltage-gated sodium channel (MpNa<sub>v</sub>) gene causes mortality in peach-potato aphid, Myzus persicae.

Tariq Kaleem K Ali Asad A Davies T G Emyr TGE Naz Erum E Naz Laila L Sohail Summar S Hou Maolin M Ullah Farman F

Scientific reports 20190328 1

Voltage-gated sodium channels (VGSC) are transmembrane proteins that generate an action potential in excitable cells and play an essential role in neuronal signaling. Since VGSCs play a crucial role in nerve transmission they have become primary targets for a broad range of commercial insecticides. RNA interference (RNAi) is a valuable reverse genetics tool used in functional genomics, but recently, it has also shown promise as a novel agent that could be used to control agricultural insect pest ...[more]

PMID: 30923355

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Project description:Aphids are amongst the most devastating sap-feeding insects of plants. Like most plant parasites, aphids require intimate associations with their host plants to gain access to nutrients. Aphid feeding induces responses such as clogging of phloem sieve elements and callose formation, which are suppressed by unknown molecules, probably proteins, in aphid saliva. Therefore, it is likely that aphids, like plant pathogens, deliver proteins (effectors) inside their hosts to modulate host cell processes, suppress plant defenses, and promote infestation. We exploited publicly available aphid salivary gland expressed sequence tags (ESTs) to apply a functional genomics approach for identification of candidate effectors from Myzus persicae (green peach aphid), based on common features of plant pathogen effectors. A total of 48 effector candidates were identified, cloned, and subjected to transient overexpression in Nicotiana benthamiana to assay for elicitation of a phenotype, suppression of the Pathogen-Associated Molecular Pattern (PAMP)-mediated oxidative burst, and effects on aphid reproductive performance. We identified one candidate effector, Mp10, which specifically induced chlorosis and local cell death in N. benthamiana and conferred avirulence to recombinant Potato virus X (PVX) expressing Mp10, PVX-Mp10, in N. tabacum, indicating that this protein may trigger plant defenses. The ubiquitin-ligase associated protein SGT1 was required for the Mp10-mediated chlorosis response in N. benthamiana. Mp10 also suppressed the oxidative burst induced by flg22, but not by chitin. Aphid fecundity assays revealed that in planta overexpression of Mp10 and Mp42 reduced aphid fecundity, whereas another effector candidate, MpC002, enhanced aphid fecundity. Thus, these results suggest that, although Mp10 suppresses flg22-triggered immunity, it triggers a defense response, resulting in an overall decrease in aphid performance in the fecundity assays. Overall, we identified aphid salivary proteins that share features with plant pathogen effectors and therefore may function as aphid effectors by perturbing host cellular processes.

Dataset Information

RNA interference-mediated knockdown of voltage-gated sodium channel (MpNa_v) gene causes mortality in peach-potato aphid, Myzus persicae.

Publications

RNA interference-mediated knockdown of voltage-gated sodium channel (MpNa<sub>v</sub>) gene causes mortality in peach-potato aphid, Myzus persicae.

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RNA interference-mediated knockdown of voltage-gated sodium channel (MpNav) gene causes mortality in peach-potato aphid, Myzus persicae.

Publications

RNA interference-mediated knockdown of voltage-gated sodium channel (MpNa<sub>v</sub>) gene causes mortality in peach-potato aphid, Myzus persicae.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

RNA interference-mediated knockdown of voltage-gated sodium channel (MpNa_v) gene causes mortality in peach-potato aphid, Myzus persicae.