¹H, ¹³C, and ¹⁵N resonance assignments of the C-terminal lobe of the human HECT E3 ubiquitin ligase ITCH.

ABSTRACT: ITCH (aka Atrophin-1-interacting protein 4) is a prominent member of the NEDD4 HECT (Homologous to E6AP C-Terminus) E3 ubiquitin ligase family that regulates numerous cellular functions including inflammatory responses through T-cell activation, cell differentiation, and apoptosis. Known intracellular targets of ITCH-dependent ubiquitylation include receptor proteins, signaling molecules, and transcription factors. The HECT C-terminal lobe of ITCH contains the conserved catalytic cysteine required for the covalent attachment of ubiquitin onto a substrate and polyubiquitin chain assembly. We report here the complete experimentally determined ¹H, ¹³C, and ¹⁵N backbone and sidechain resonance assignments for the HECT C-terminal lobe of ITCH (residues 784-903)

SUBMITTER: Beasley SA 

PROVIDER: S-EPMC6439258 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature