Enhanced and controlled chromatin extraction from FFPE tissues and the application to ChIP-seq.

ABSTRACT:

Background

Epigenetic dysregulation is involved in the etiology and progression of various human diseases. Formalin-fixed paraffin-embedded (FFPE) samples represent the gold standard for archiving pathology samples, and thus FFPE samples are a major resource of samples in clinical research. However, chromatin-based epigenetic assays in the clinical settings are limited to fresh or frozen samples, and are hampered by low chromatin yield in FFPE samples due to the lack of a reliable and efficient chromatin preparation method. Here, we introduce a new chromatin extraction method from FFPE tissues (Chrom-EX PE) for chromatin-based epigenetic assays.

Results

During rehydration of FFPE tissues, applying a tissue-level cross-link reversal into the deparaffinized tissue at 65 °C dra

SUBMITTER: Zhong J 

PROVIDER: S-EPMC6440302 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature