Structural switching electrochemical DNA aptasensor for the rapid diagnosis of tuberculous meningitis.

ABSTRACT:

Background

Tuberculous meningitis (TBM) is the most devastating manifestation of extra-pulmonary tuberculosis. About 33% of TBM patients die due to very late diagnosis of the disease. Conventional diagnostic methods based on signs and symptoms, cerebrospinal fluid (CSF) smear microscopy or liquid culture suffer from either poor sensitivity or long turnaround time (up to 8 weeks). Therefore, in order to manage the disease efficiently, there is an urgent and unmet need for a rapid and reliable diagnostic test.

Methods

In the current study, to address the diagnostic challenge of TBM, a highly rapid and sensitive structural switching electrochemical aptasensor was developed by combining the electrochemical property of methylene blue (MB) with the molecular recognition ability of

SUBMITTER: Das R 

PROVIDER: S-EPMC6440448 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature