Liposomes targeted to MHC-restricted antigen improve drug delivery and antimelanoma response.

ABSTRACT:

Purpose

Melanoma is the most aggressive form of skin cancer. Chemotherapy at a late stage fails due to low accumulation in tumors, indicating the need for targeted therapy.

Materials and methods

To increase drug uptake by tumor cells, we have targeted doxorubicin-containing liposomes using a T-cell receptor (TCR)-like antibody (scFv G8 and Hyb3) directed against melanoma antigen A1 (MAGE-A1) presented by human leukocyte antigen A1 (M1/A1). With the use of flow cytometry and confocal microscopy, we have tested our formulation in vitro. In vivo pharmacokinetics was done in tumor-free nu/nu mice, while biodistribution and efficacy study was done in nu/nu mice xenograft.

Results

We demonstrated two to five times higher binding and internalization of these immunoliposomes

SUBMITTER: Saeed M 

PROVIDER: S-EPMC6440454 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature