The Utah Protocol for Postmortem Eye Phenotyping and Molecular Biochemical Analysis.

ABSTRACT:

Purpose

Current understanding of local disease pathophysiology in AMD is limited. Analysis of the human disease-affected tissue is most informative, as gene expression, expressed quantitative trait loci, microenvironmental, and epigenetic changes can be tissue, cell type, and location specific. Development of a novel translational treatment and prevention strategies particularly for earlier forms of AMD are needed, although access to human ocular tissue analysis is challenging. We present a standardized protocol to study rapidly processed postmortem donor eyes for molecular biochemical and genomic studies.

Methods

We partnered with the Utah Lions Eye Bank to obtain donor human eyes, blood, and vitreous, within 6 hours postmortem. Phenotypic analysis was performed using spect

SUBMITTER: Owen LA 

PROVIDER: S-EPMC6440527 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature