Metformin induces the AP-1 transcription factor network in normal dermal fibroblasts.

ABSTRACT: Metformin is a widely-used treatment for type 2 diabetes and is reported to extend health and lifespan as a caloric restriction (CR) mimetic. Although the benefits of metformin are well documented, the impact of this compound on the function and organization of the genome in normal tissues is unclear. To explore this impact, primary human fibroblasts were treated in culture with metformin resulting in a significant decrease in cell proliferation without evidence of cell death. Furthermore, metformin induced repositioning of chromosomes 10 and 18 within the nuclear volume indicating altered genome organization. Transcriptome analyses from RNA sequencing datasets revealed that alteration in growth profiles and chromosome positioning occurred concomitantly with changes in gene expression prof

SUBMITTER: Gillespie ZE 

PROVIDER: S-EPMC6441003 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature