Direct differentiation of bone marrow mononucleated cells into insulin producing cells using pancreatic β-cell-derived components.

ABSTRACT: Transplantation of stem cell-derived insulin producing cells (IPCs) has been proposed as an alternative to islet transplantation for the treatment of diabetes mellitus. However, current IPC differentiation protocols are focused on generating functional cells from the pluripotent stem cells and tend to rely on multistep, long-term exposure to various exogenous factors. In this study, we addressed the observation that under stress, pancreatic β-cells release essential components that direct the differentiation of the bone marrow nucleated cells (BMNCs) into IPCs. Without any supplementation with known differentiation-inducing factors, IPCs can be generated from BMNCs by in vitro priming for 6 days with conditioned media (CM) from the β-cells. In vitro primed BMNCs expressed the β-cell-specif

SUBMITTER: Oh JE 

PROVIDER: S-EPMC6441031 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature