Proximal and distal control for ligand binding in neuroglobin: role of the CD loop and evidence for His64 gating.

ABSTRACT: Neuroglobin (Ngb) is predominantly expressed in neurons of the central and peripheral nervous systems and it clearly seems to be involved in neuroprotection. Engineering Ngb to observe structural and dynamic alterations associated with perturbation in ligand binding might reveal important structural determinants, and could shed light on key features related to its mechanism of action. Our results highlight the relevance of the CD loop and of Phe106 as distal and proximal controls involved in ligand binding in murine neuroglobin. We observed the effects of individual and combined mutations of the CD loop and Phe106 that conferred to Ngb higher CO binding velocities, which we correlate with the following structural observations: the mutant F106A shows, upon CO binding, a reduced heme sliding

SUBMITTER: Exertier C 

PROVIDER: S-EPMC6441039 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature