Multiplex imaging relates quantal glutamate release to presynaptic Ca²⁺ homeostasis at multiple synapses in situ.

ABSTRACT: Information processing by brain circuits depends on Ca²⁺-dependent, stochastic release of the excitatory neurotransmitter glutamate. Whilst optical glutamate sensors have enabled detection of synaptic discharges, understanding presynaptic machinery requires simultaneous readout of glutamate release and nanomolar presynaptic Ca²⁺ in situ. Here, we find that the fluorescence lifetime of the red-shifted Ca²⁺ indicator Cal-590 is Ca²⁺-sensitive in the nanomolar range, and employ it in combination with green glutamate sensors to relate quantal neurotransmission to presynaptic Ca²⁺ kinetics. Multiplexed imaging of individual and multiple synapses in identified axonal circuits reveals that glutamate release efficacy, but not its short-term pl

SUBMITTER: Jensen TP 

PROVIDER: S-EPMC6441074 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature