A sensitive and robust UPLC-MS/MS method for quantitation of estrogens and progestogens in human serum.

ABSTRACT:

Objective

With the widespread use of sex-steroid hormones in contraceptives and hormone replacement therapy, there is an increasing need for reliable analytical methods. We report the development of a sensitive and robust UPLC-MS/MS method for quantitation of both endogenous and synthetic sex-steroid hormones in human serum.

Study design

We developed and validated a UPLC-MS/MS method to quantify progestogens (etonogestrel, levonorgestrel, medroxyprogesterone acetate, norethindrone, progesterone) and estrogens (estradiol and ethinyl estradiol) with good accuracy, high sensitivity, and excellent robustness. We then applied the method to the analysis of sex-steroid hormones in serum from 451 clinical research participants.

Results

Each UPLC-MS/MS analysis was 6.5 min. Th

SUBMITTER: Zhang J 

PROVIDER: S-EPMC6441366 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature