DNA methylation in nasal epithelium, atopy, and atopic asthma in children: a genome-wide study.

ABSTRACT:

Background

Epigenetic mechanisms could alter the airway epithelial barrier and ultimately lead to atopic diseases such as asthma. We aimed to identify DNA methylation profiles that are associated with-and could accurately classify-atopy and atopic asthma in school-aged children.

Methods

We did a genome-wide study of DNA methylation in nasal epithelium and atopy or atopic asthma in 483 Puerto Rican children aged 9-20 years, recruited using multistage probability sampling. Atopy was defined as at least one positive IgE (≥0·35 IU/mL) to common aeroallergens, and asthma was defined as a physician's diagnosis plus wheeze in the previous year. Significant (false discovery rate p<0·01) methylation signals were correlated with gene expression, and top CpGs were validated by pyrosequ

SUBMITTER: Forno E 

PROVIDER: S-EPMC6441380 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature