Interrogation of human hematopoiesis at single-cell and single-variant resolution.

ABSTRACT: Widespread linkage disequilibrium and incomplete annotation of cell-to-cell state variation represent substantial challenges to elucidating mechanisms of trait-associated genetic variation. Here we perform genetic fine-mapping for blood cell traits in the UK Biobank to identify putative causal variants. These variants are enriched in genes encoding proteins in trait-relevant biological pathways and in accessible chromatin of hematopoietic progenitors. For regulatory variants, we explore patterns of developmental enhancer activity, predict molecular mechanisms, and identify likely target genes. In several instances, we localize multiple independent variants to the same regulatory element or gene. We further observe that variants with pleiotropic effects preferentially act in common progenit

SUBMITTER: Ulirsch JC 

PROVIDER: S-EPMC6441389 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature