JAM3 functions as a novel tumor suppressor and is inactivated by DNA methylation in colorectal cancer.

ABSTRACT:

Purpose

JAM3, an adhesion and transmigration regulatory element, is abundantly expressed in intestinal epithelial cells. However, its expression and function in colorectal cancer (CRC) remain unknown. In this study, we explored its epigenetic mechanism and biological role in CRC.

Patients and methods

Bioinformatics analysis was used to analyze the expression and methylation level of JAM3 in CRC. Methylation and expression status of JAM3 were then validated by quantitative methylation-specific PCR (qMSP) and quantitative PCR in tissues, plasma samples, and cell lines. Flow cytometry, Western blot, transwell, siRNA, colony formation, and transfection were used to evaluate the biological function of JAM3.

Results

We initially found that JAM3 was frequently methylated and

SUBMITTER: Zhou D 

PROVIDER: S-EPMC6441464 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature