TRIM31 promotes glioma proliferation and invasion through activating NF-κB pathway.

ABSTRACT:

Background

Glioma is the most lethal primary brain tumor, the survival rate still isn't improved in the past decades. It's essential to study the regulatory mechanism of glioma progression, hoping to find new therapy targets or methods. The family of tripartite motif (TRIM) containing proteins are E3 ubiquitination ligases, which play critical role in various tumor progression.

Methods

Cell proliferation and invasion were analyzed by colony formation assay, soft agar growth assay, BrdU incorporation assay and transwell invasion assay. Luciferase reporter analysis was used to analyze NF-κB pathway activity.

Results

We found TRIM31 was upregulated in glioma cells and tissues, its overexpression significantly promoted glioma cell proliferation and invasion, while its kno

SUBMITTER: Zhou L 

PROVIDER: S-EPMC6441556 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature