The complexity of the Fragaria x ananassa (octoploid) transcriptome by single-molecule long-read sequencing.

ABSTRACT: Strawberry (Fragaria x ananassa) is an allopolyploid species with diverse and complex transcripts. The regulatory mechanisms of fruit development and maturation have been extensively studied; however, little is known about the signaling mechanisms that direct this process in octoploid strawberry (Fragaria x ananassa). Here, we used long-read sequencing (LRS) technology and RNA-seq analysis to investigate the diversity and complexity of the polyploid transcriptome and differentially expressed transcripts along four successive fruit developmental stages of cultivated strawberry. We obtained a reference transcriptome with 119,897 unique full-length isoforms, including 2017 new isoforms and 2510 long noncoding RNAs. Based on the genome of the plausible progenitor (<

SUBMITTER: Yuan H 

PROVIDER: S-EPMC6441658 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature