The degree of major histocompatibility complex matching between purebred Maltese and mongrel dogs using microsatellite markers.

ABSTRACT: Long-term maintenance of transplanted organs is one of the major factors that increases survival time of recipients. Although obtaining a major histocompatibility complex (MHC)-matched donor with the recipient is essential for successful organ transplantation, there have been limited reports on MHC matching between dogs. In this study, we analyzed the canine MHC matching rates using Maltese, one of the most popular purebred dogs, and mongrel dogs in Korea. Genomic DNA was extracted from blood leukocytes and DNA was amplified by polymerase chain reaction with primers specific to MHC microsatellite markers. The MHC matching degree was confirmed by the microsatellite markers using polyacrylamide gel electrophoresis. The MHC matching rates of each donor-recipient groups including Maltese-Malte

SUBMITTER: Kwak HH 

PROVIDER: S-EPMC6441805 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature