A GBM-like V-ATPase signature directs cell-cell tumor signaling and reprogramming via large oncosomes.

ABSTRACT:

Background

The V-ATPase proton pump controls acidification of intra and extra-cellular milieu in both physiological and pathological conditions. We previously showed that some V-ATPase subunits are enriched in glioma stem cells and in patients with poor survival. In this study, we investigated how expression of a GBM-like V-ATPase pump influences the non-neoplastic brain microenvironment.

Methods

Large oncosome (LO) vesicles were isolated from primary glioblastoma (GBM) neurospheres, or from patient sera, and co-cultured with primary neoplastic or non-neoplastic brain cells. LO transcript and protein contents were analyzed by qPCR, immunoblotting and immunogold staining. Activation of pathways in recipient cells was determined at gene and protein expression levels. V-ATPase

SUBMITTER: Bertolini I 

PROVIDER: S-EPMC6441844 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature