Integrated epigenomic profiling reveals endogenous retrovirus reactivation in renal cell carcinoma.

ABSTRACT:

Background

Transcriptional dysregulation drives cancer formation but the underlying mechanisms are still poorly understood. Renal cell carcinoma (RCC) is the most common malignant kidney tumor which canonically activates the hypoxia-inducible transcription factor (HIF) pathway. Despite intensive study, novel therapeutic strategies to target RCC have been difficult to develop. Since the RCC epigenome is relatively understudied, we sought to elucidate key mechanisms underpinning the tumor phenotype and its clinical behavior.

Methods

We performed genome-wide chromatin accessibility (DNase-seq) and transcriptome profiling (RNA-seq) on paired tumor/normal samples from 3 patients undergoing nephrectomy for removal of RCC. We incorporated publicly available data on HIF binding (ChI

SUBMITTER: Siebenthall KT 

PROVIDER: S-EPMC6441874 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature