Co-activation of macrophages and T cells contribute to chronic GVHD in human IL-6 transgenic humanised mouse model.

ABSTRACT:

Background

Graft-versus host disease (GVHD) is a complication of stem cell transplantation associated with significant morbidity and mortality. Non-specific immune-suppression, the mainstay of treatment, may result in immune-surveillance dysfunction and disease recurrence.

Methods

We created humanised mice model for chronic GVHD (cGVHD) by injecting cord blood (CB)-derived human CD34⁺CD38^-CD45RA^- haematopoietic stem/progenitor cells (HSPCs) into hIL-6 transgenic NOD/SCID/Il2rgKO (NSG) newborns, and compared GVHD progression with NSG newborns receiving CB CD34^- cells mimicking acute GVHD. We characterised human immune cell subsets, target organ infiltration, T-cell repertoire (TCR) and transcriptome in the humanised mice.

Finding

SUBMITTER: Ono R 

PROVIDER: S-EPMC6441951 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature