Imaging flow cytometry facilitates multiparametric characterization of extracellular vesicles in malignant brain tumours.

ABSTRACT: Cells release heterogeneous nano-sized vesicles either as exosomes, being derived from endosomal compartments, or through budding from the plasma membrane as so-called microvesicles, commonly referred to as extracellular vesicles (EVs). EVs are known for their important roles in mammalian physiology and disease pathogenesis and provide a potential biomarker source in cancer patients. EVs are generally often analysed in bulk using Western blotting or by bead-based flow-cytometry or, with limited parameters, through nanoparticle tracking analysis. Due to their small size, single EV analysis is technically highly challenging. Here we demonstrate imaging flow cytometry (IFCM) to be a robust, multiparametric technique that allows analysis of single EVs and the discrimination of distinct EV subp

SUBMITTER: Ricklefs FL 

PROVIDER: S-EPMC6442086 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature