DNMT1 in Six2 Progenitor Cells Is Essential for Transposable Element Silencing and Kidney Development.

ABSTRACT:

Background

Cytosine methylation of regulatory regions, such as promoters and enhancers, plays a key role in regulating gene expression, however, its role in kidney development has not been analyzed.

Methods

To identify functionally important epigenome-modifying enzymes and genome regions where methylation modifications are functionally important for kidney development, we performed genome-wide methylation analysis, expression profiling, and systematic genetic targeting of DNA methyltransferases (Dnmt1, Dnmt3a, and Dnmt3b) and Ten-eleven translocation methylcytosine hydroxylases (Tet2) in nephron progenitor cells (Six2^Cre) in mice.

Results

Genome-wide methylome analysis indicated dynamic changes on promoters and enhancers d

SUBMITTER: Li SY 

PROVIDER: S-EPMC6442333 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature