The RNA-Protein Interactome of Differentiated Kidney Tubular Epithelial Cells.

ABSTRACT:

Background

RNA-binding proteins (RBPs) are fundamental regulators of cellular biology that affect all steps in the generation and processing of RNA molecules. Recent evidence suggests that regulation of RBPs that modulate both RNA stability and translation may have a profound effect on the proteome. However, regulation of RBPs in clinically relevant experimental conditions has not been studied systematically.

Methods

We used RNA interactome capture, a method for the global identification of RBPs to characterize the global RNA-binding proteome (RBPome) associated with polyA-tailed RNA species in murine ciliated epithelial cells of the inner medullary collecting duct. To study regulation of RBPs in a clinically relevant condition, we analyzed hypoxia-associated changes of the

SUBMITTER: Ignarski M 

PROVIDER: S-EPMC6442340 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature