Chromatin architecture reorganization during neuronal cell differentiation in Drosophila genome.

ABSTRACT: The organization of the genome into topologically associating domains (TADs) was shown to have a regulatory role in development and cellular function, but the mechanism involved in TAD establishment is still unclear. Here, we present the first high-resolution contact map of Drosophila neuronal cells (BG3) and identify different classes of TADs by comparing this to genome organization in embryonic cells (Kc167). We find that only some TADs are conserved in both cell lines, whereas the rest are cell-type-specific. This is supported by a change in the enrichment of architectural proteins at TAD borders, with BEAF-32 present in embryonic cells and CTCF in neuronal cells. Furthermore, we observe strong divergent transcription, together with RNA Polymerase II occupancy and an increase in

SUBMITTER: Chathoth KT 

PROVIDER: S-EPMC6442379 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature