Expression and secretion of a lytic polysaccharide monooxygenase by a fast-growing cyanobacterium.

ABSTRACT:

Background

Cyanobacteria have the potential to become next-generation cell factories due to their ability to use CO₂, light and inorganic nutrients to produce a range of biomolecules of commercial interest. Synechococcus elongatus UTEX 2973, in particular, is a fast-growing, genetically tractable, cyanobacterium that has garnered attention as a potential biotechnological chassis. To establish this unique strain as a host for heterologous protein production, we aimed to demonstrate expression and secretion of the industrially relevant TfAA10A, a lytic polysaccharide monooxygenase from the Gram-positive bacterium Thermobifida fusca.

Results

Two variations of TfAA10A were successfully expressed in S. elongatus UTEX 2973: One contain

SUBMITTER: Russo DA 

PROVIDER: S-EPMC6442416 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature