Vitamin D Receptor Gene Polymorphism: An Important Predictor of Arthritis Development.

ABSTRACT: Vitamin D is an anti-inflammatory molecule and has a role in prevention of arthritis development. Biologically active form 1, 25(OH)₂D3 of vitamin D can only exert its action after binding its definite vitamin D receptor encoded by VDR gene. VDR gene polymorphism leads to dysfunctioning of 1, 25(OH)₂D₃ ultimately disease onset. The purpose of current study was to evaluate the effect of vitamin D level and VDR gene polymorphism on rheumatoid arthritis and osteoarthritis. Blood samples were collected from case and control after taking written consent. Serum was separated and vitamin D level as determined from each sample by ELISA. DNA was extracted from each blood sample and amplified by using gene specific primers. Genotyping was performed by Sangers sequenc

SUBMITTER: Mukhtar M 

PROVIDER: S-EPMC6442488 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature