Multiplexed profiling of single-cell extracellular vesicles secretion.

ABSTRACT: Extracellular vesicles (EVs) are important intercellular mediators regulating health and diseases. Conventional methods for EV surface marker profiling, which was based on population measurements, masked the cell-to-cell heterogeneity in the quantity and phenotypes of EV secretion. Herein, by using spatially patterned antibody barcodes, we realized multiplexed profiling of single-cell EV secretion from more than 1,000 single cells simultaneously. Applying this platform to profile human oral squamous cell carcinoma (OSCC) cell lines led to a deep understanding of previously undifferentiated single-cell heterogeneity underlying EV secretion. Notably, we observed that the decrement of certain EV phenotypes (e.g., ^CD63+EV) was associated with the invasive feature of both OSCC cell l

SUBMITTER: Ji Y 

PROVIDER: S-EPMC6442581 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature