Discovery of novel carbohydrate-active enzymes through the rational exploration of the protein sequences space.

ABSTRACT: Over the last two decades, the number of gene/protein sequences gleaned from sequencing projects of individual genomes and environmental DNA has grown exponentially. Only a tiny fraction of these predicted proteins has been experimentally characterized, and the function of most proteins remains hypothetical or only predicted based on sequence similarity. Despite the development of postgenomic methods, such as transcriptomics, proteomics, and metabolomics, the assignment of function to protein sequences remains one of the main challenges in modern biology. As in all classes of proteins, the growing number of predicted carbohydrate-active enzymes (CAZymes) has not been accompanied by a systematic and accurate attribution of function. Taking advantage of the CAZy database, which groups CAZyme

SUBMITTER: Helbert W 

PROVIDER: S-EPMC6442616 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature