Point centromere activity requires an optimal level of centromeric noncoding RNA.

ABSTRACT: In budding yeast, which possesses simple point centromeres, we discovered that all of its centromeres express long noncoding RNAs (cenRNAs), especially in S phase. Induction of cenRNAs coincides with CENP-A^Cse4 loading time and is dependent on DNA replication. Centromeric transcription is repressed by centromere-binding factor Cbf1 and histone H2A variant H2A.Z^Htz1 Deletion of CBF1 and H2A.Z^HTZ1 results in an up-regulation of cenRNAs; an increased loss of a minichromosome; elevated aneuploidy; a down-regulation of the protein levels of centromeric proteins CENP-A^Cse4, CENP-A chaperone HJURP^Scm3, CENP-C^Mif2, Survivin^Bir1, and INCENP^Sli15; and a reduced chromatin localization of CENP-

SUBMITTER: Ling YH 

PROVIDER: S-EPMC6442628 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature