Targeting the functional interplay between endoplasmic reticulum oxidoreductin-1α and protein disulfide isomerase suppresses the progression of cervical cancer.

ABSTRACT:

Background

Endoplasmic reticulum (ER) oxidoreductin-1α (Ero1α) and protein disulfide isomerase (PDI) constitute the pivotal pathway of oxidative protein folding, and are highly expressed in many cancers. However, whether targeting the functional interplay between Ero1α and PDI could be a new approach for cancer therapy remains unknown.

Methods

We performed wound healing assays, transwell migration and invasion assays and xenograft assays to assess cell migration, invasion and tumorigenesis; gel filtration chromatography, oxygen consumption assay and in cells folding assays were used to detect Ero1α-PDI interaction and Ero1α oxidase activity.

Findings

Here, we report that elevated expression of Ero1α is correlated with poor prognosis in human cervical cancer. Knockout

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC6443025 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature