Proteomics based identification of KDM5 histone demethylases associated with cardiovascular disease.

ABSTRACT:

Background

The increased prevalence of cardiovascular disease (CVD) indicates a demand for novel therapeutic approaches. Proteome analysis of vascular tissues from animal models and humans with CVD could lead to the identification of novel druggable targets.

Methods

LC-MS/MS analysis of thoracic aortas from three mouse models of non-diabetic and diabetic (streptozotocin (STZ)-induced) atherosclerosis followed by bioinformatics/pathway analysis was performed. Selected findings were confirmed by proteomics analysis of human vessels from patients with CVD as well as in vitro studies (migration, proliferation, angiogenesis assays) using endothelial (HUVEC) cells.

Findings

Comparative tissue proteomics of low density lipoprotein receptor deficient (Ldlr-/-) and diabetic Ld

SUBMITTER: Mokou M 

PROVIDER: S-EPMC6443027 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature