Dissecting alterations in human CD8+ T cells with aging by high-dimensional single cell mass cytometry.

ABSTRACT: We investigated the effect of aging on the multi-dimensional characteristics and heterogeneity of human peripheral CD8⁺ T cells defined by the expression of a set of molecules at the single cell level using the recently developed mass cytometry or Cytometry by Time-Of-Flight (CyTOF) and computational algorithms. CD8⁺ T cells of young and older adults had differential expression of molecules, especially those related to cell activation and migration, permitting the clustering of young and older adults through an unbiased approach. The changes in the expression of individual molecules were collectively reflected in the altered high-dimensional profiles of CD8⁺ T cells in older adults as visualized by the dimensionality reduction analysis tools principal compo

SUBMITTER: Shin MS 

PROVIDER: S-EPMC6443094 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature