The adverse effect of gentamicin on cell metabolism in three cultured mammary cell lines: "Are cell culture data skewed?"

ABSTRACT: Many cells are cultured in media that contains an antibiotic to prevent bacterial contamination. Mycoplasma and other bacterial contamination is a serious problem for those involved in cell culture. Antibiotics in the media helps prevent this contamination and make life easier for the investigators; as performing cell culture experiments in antibiotic free media is difficult and requires vigorous sterile technique. There are many reports of antibiotics causing mitochondrial damage. In this study, we tested the effect of gentamicin in culture media on human mammary epithelial MCF-12A and breast cancer MCF-7 and MDA-MB-231 cell lines by real time PCR, immunofluorescent microscopy, lactate assay, DNA damage assay. We found that the addition of gentamicin in media upregulated the gene expressi

SUBMITTER: Elliott RL 

PROVIDER: S-EPMC6443165 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature