A protocol to count Cryptosporidium oocysts by flow cytometry without antibody staining.

ABSTRACT: Cryptosporidiosis caused by the protozoan parasites Cryptosporidium hominis and C. parvum, threatens the lives of young children in developing countries. In veterinary medicine, C. parvum causes life-threatening diarrhea and dehydration in newborn dairy calves. Protocols to detect Cryptosporidium spp. oocysts using flow cytometry have been reported; however, these protocols use antibodies against the parasite and typically focus on detection of oocysts, not quantification. These techniques are not well-suited for studies that generate large variations in oocyst burdens because the amount of antibody required is proportional to the number of oocysts expected in samples. Also, oocysts are lost in washes in the staining protocol, reducing accuracy of oocyst counts. Moreover, these protocols r

SUBMITTER: Sonzogni-Desautels K 

PROVIDER: S-EPMC6443187 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature