Fragmentation Through Polymerization (FTP): A new method to fragment DNA for next-generation sequencing.

ABSTRACT: Fragmentation of DNA is the very important first step in preparing nucleic acids for next-generation sequencing. Here we report a novel Fragmentation Through Polymerization (FTP) technique, which is a simple, robust, and low-cost enzymatic method of fragmentation. This method generates double-stranded DNA fragments that are suitable for direct use in NGS library construction and allows the elimination of the additional step of reparation of DNA ends.

SUBMITTER: Ignatov KB 

PROVIDER: S-EPMC6443234 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature