MiR-4739 mediates pleural fibrosis by targeting bone morphogenetic protein 7.

ABSTRACT:

Background

Pleural fibrosis is defined as excessive depositions of matrix components that result in pleural tissue architecture destruction and dysfunction. In severe cases, the progression of pleural fibrosis leads to lung entrapment, resulting in dyspnea and respiratory failure. However, the mechanism of pleural fibrosis is poorly understood.

Methods

miR-4739 levels were detected by miRNA array and real-time PCR. Real-time PCR, western blotting and immunofluorescence were used to identify the expression profile of indicators related to fibrosis. Target gene of miR-4739 and promoter activity assay was measured by using dual-luciferase reporter assay system. In vivo, pleural fibrosis was evaluated by Masson staining and miR-4739 level was detected by In situ hybridization hi

SUBMITTER: Wang M 

PROVIDER: S-EPMC6443597 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature