A temporary indwelling intravascular aphaeretic system for in vivo enrichment of circulating tumor cells.

ABSTRACT: Circulating tumor cells (CTCs) have become an established biomarker for prognosis in patients with various carcinomas. However, current ex vivo CTC isolation technologies rely on small blood volumes from a single venipuncture limiting the number of captured CTCs. This produces statistical variability and inaccurate reflection of tumor cell heterogeneity. Here, we describe an in vivo indwelling intravascular aphaeretic CTC isolation system to continuously collect CTCs directly from a peripheral vein. The system returns the remaining blood products after CTC enrichment, permitting interrogation of larger blood volumes than classic phlebotomy specimens over a prolonged period of time. The system is validated in canine models showing capability to screen 1-2% of the entire blood over 2 h. Our

SUBMITTER: Kim TH 

PROVIDER: S-EPMC6443676 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature