Effect of cell culture density on dental pulp-derived mesenchymal stem cells with reference to osteogenic differentiation.

ABSTRACT: Dental pulp stem cells (DPSCs) are a good source for tissue regeneration, however, the number of DPSCs in the pulp tissue is limited. Cell propagation is essential for tissue engineering using DPSCs and the cell culture conditions may affect the properties of DPSCs. The purpose of this study was to analyze the effect of cell culture condition, especially dense culture condition, on the property and differentiation pathway of DPSCs. We cultured DPSCs under sparse (sDPSCs; 5 × 10³ cells/cm²) or dense (dDPSCs; 1 × 10⁵ cells/cm²) conditions for 4 days and compared their properties. The populations of CD73⁺ and CD105⁺ cells were significantly decreased in dDPSCs. Both groups showed multi-differentiation potential, but mineralized

SUBMITTER: Noda S 

PROVIDER: S-EPMC6443725 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature