An optimized isolation protocol yields high-quality RNA from cassava tissues (Manihot esculenta Crantz).
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ABSTRACT:
SUBMITTER: Behnam B
PROVIDER: S-EPMC6443859 | biostudies-literature | 2019 Apr
REPOSITORIES: biostudies-literature
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An optimized isolation protocol yields high-quality RNA from cassava tissues (<i>Manihot esculenta</i> Crantz).
Behnam Babak B Bohorquez-Chaux Adriana A Castaneda-Mendez Oscar Fernando OF Tsuji Hiroyuki H Ishitani Manabu M Becerra Lopez-Lavalle Luis Augusto LA
FEBS open bio 20190220 4
We developed and modified a precise, rapid, and reproducible protocol isolating high-quality RNA from tissues of multiple varieties of cassava plants (<i>Manihot esculenta</i> Crantz). The resulting method is suitable for use in mini, midi, and maxi preparations and rapidly achieves high total RNA yields (170-600 μg·g<sup>-1</sup>) using low-cost chemicals and consumables and with minimal contamination from polysaccharides, polyphenols, proteins, and other secondary metabolites. In particular, < ...[more]
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