Piscine orthoreovirus sequences in escaped farmed Atlantic salmon in Washington and British Columbia.

ABSTRACT:

Background

Piscine orthoreovirus (PRV) is an emergent virus in salmon aquaculture belonging to the family Reoviridae. PRV is associated with a growing list of pathological conditions including heart and skeletal inflammation (HSMI) of farmed Atlantic salmon. Despite widespread PRV infection in commercially farmed Atlantic salmon, information on PRV prevalence and on the genetic sequence variation of PRV in Atlantic salmon on the north Pacific Coast is limited.

Methods

Feral Atlantic salmon caught in Washington State and British Columbia following a large containment failure at a farm in northern Puget Sound were sampled. Fish tissues were tested for PRV by RT-qPCR assay for segment L1 and conventional RT-PCR for PRV segment S1. The PCR products were sequenced and their relat

SUBMITTER: Kibenge MJT 

PROVIDER: S-EPMC6444584 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature