Multi-analyte biosensor interface for real-time monitoring of 3D microtissue spheroids in hanging-drop networks.

ABSTRACT: Microfluidics is becoming a technology of growing interest for building microphysiological systems with integrated read-out functionalities. Here we present the integration of enzyme-based multi-analyte biosensors into a multi-tissue culture platform for 'body-on-a-chip' applications. The microfluidic platform is based on the technology of hanging-drop networks, which is designed for the formation, cultivation, and analysis of fluidically interconnected organotypic spherical three-dimensional (3D) microtissues of multiple cell types. The sensor modules were designed as small glass plug-ins featuring four platinum working electrodes, a platinum counter electrode, and an Ag/AgCl reference electrode. They were placed directly into the ceiling substrate from which the hanging drops that host t

SUBMITTER: Misun PM 

PROVIDER: S-EPMC6444714 | biostudies-literature | 2016

REPOSITORIES: biostudies-literature