A new hyperpolarized ¹³C ketone body probe reveals an increase in acetoacetate utilization in the diabetic rat heart.

ABSTRACT: Emerging studies have recently shown the potential importance of ketone bodies in cardio-metabolic health. However, techniques to determine myocardial ketone body utilization in vivo are lacking. In this work, we developed a novel method to assess myocardial ketone body utilization in vivo using hyperpolarized [3-¹³C]acetoacetate and investigated the alterations in myocardial ketone body metabolism in diabetic rats. Within a minute upon injection of [3-¹³C]acetoacetate, the production of [5-¹³C]glutamate and [1-¹³C] acetylcarnitine can be observed real time in vivo. In diabetic rats, the production of [5-¹³C]glutamate was elevated compared to controls, while [1-¹³C]acetylcarnitine was not different. This suggests an increase

SUBMITTER: Abdurrachim D 

PROVIDER: S-EPMC6445118 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature