Factors influencing meiotic recombination revealed by whole-genome sequencing of single sperm.

ABSTRACT: Recombination is critical to meiosis and evolution, yet many aspects of the physical exchange of DNA via crossovers remain poorly understood. We report an approach for single-cell whole-genome DNA sequencing by which we sequenced 217 individual hybrid mouse sperm, providing a kilobase-resolution genome-wide map of crossovers. Combining this map with molecular assays measuring stages of recombination, we identified factors that affect crossover probability, including PRDM9 binding on the non-initiating template homolog and telomere proximity. These factors also influence the time for sites of recombination-initiating DNA double-strand breaks to find and engage their homologs, with rapidly engaging sites more likely to form crossovers. We show that chromatin environment on the template homol

SUBMITTER: Hinch AG 

PROVIDER: S-EPMC6445350 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature