Pooling skin swabs does not inhibit qPCR detection of amphibian chytrid infection.

ABSTRACT: Immediate and reliable pathogen detection in large numbers of samples is essential in wildlife disease monitoring and is often realized by DNA-based techniques. Pooling samples increases processing efficiency and reduces processing costs, and has been suggested as a viable technique for quantitative PCR detection of fungal amphibian pathogens of the genus Batrachochytrium. For these fungi, this diagnostic method has been validated by in vitro set ups that provided controlled test conditions but did not take into account potential effects from amphibian skin compounds (e.g. skin secretions and Microbiota) on the approach. Some of these skin compounds are known to cause PCR inhibition in single sample applications and could lead to false negative reactions and thereby hamper pathogen detecti

SUBMITTER: Sabino-Pinto J 

PROVIDER: S-EPMC6445426 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature